| Problem |
Possible
Cause |
Solution |
|
Calibration
Curve
• 1000
ng/mL Calibrator
(calculated) is out of range
• 100
ng/mL Calibrator
(calculated) is out of range
• Correlation
coefficient
(r2) is out of range |
|
Improper identification of Calibrator
wells in Calculator |
Correct numeric value(s) and recalculate
the Calibration Curve |
Calibrators not mixed
thoroughly
|
Repeat Assay Part 3
using new Calibrator Panel |
| Cross-contamination
between wells |
Repeat Assay Part 3 |
| Deterioration of Calibrators |
Repeat Assay Part 3
using new Calibrator Panel |
| Contamination of Calibrators |
Repeat Assay Part 3
using new Calibrator Panel |
Improper volume of ATP Calibrator dispensed into wells
|
| Luminometer related problem |
|
Repeat Assay Part 3
|
| Refer to luminometer manufacturer’s
recommendations; freeze Assay Plate strips |
|
|
Non-Stimulated
Control Specimen
• ATP
result is >60 ng/mL |
|
Blood was diluted improperly |
Refer to procedure for
proper protocol; repeat test using fresh specimens |
An anti-coagulant other
than
sodium heparin was used |
Repeat test using fresh
specimens
collected into a sodium heparin tube |
| Cross contamination
of wells |
Decrease setting of
plate shaker to allow vigorous shaking without splashing |
| Calibration Curve is
invalid |
Refer to first section
of this table |
| Improper volume of lysate
transferred into Measurement Plate |
Repeat Assay Part 3 |
| Improper volume of Calibrators
dispensed into Measurement Plate |
Repeat Assay Part 3 |
| Deterioration of Calibrators |
Repeat Assay Part 3
using new Calibrator Panel |
| The ATP value may be
accurate for the individual chosen as the control |
Choose another source
for control specimen and repeat test using fresh
specimens |
|
Stimulated
Control Specimen
• ATP
result is <240 ng/mL |
|
Blood specimen is not
fresh |
Repeat test using fresh
specimens |
| Blood specimen was subjected
to temperature changes or excessive mixing |
Repeat test using fresh
specimens |
| An anti-coagulant other
than sodium heparin was used |
Repeat test using fresh
specimens collected into a sodium heparin tube |
| Blood was diluted improperly |
Refer to procedure for
proper protocol; repeat test using fresh specimens |
| Activity of Stimulant
has decreased |
Repeat test using fresh
specimens and use a new vial of Stimulant |
| Improper volumes of
specimen, Stimulant, or other reagents were used |
Refer to procedure for
proper protocol; repeat test using fresh specimens |
| Calibration curve is
invalid |
Refer to first section
of this table |
| Improper volume of lysate
transferred into Measurement Plate |
Repeat Assay Part 3 |
| Improper volume of Calibrators
dispensed into Measurement Plate |
Repeat Assay Part 3 |
| Vacuum strength is too
high, causing loss of Dynabeads® |
Adjust the level of
the vacuum strength to achieve a slow, gentle aspiration |
| Plate shaker is not
vigorous enough for optimal mixing |
Increase setting of
plate shaker to allow vigorous shaking without splashing |
| Incubation
time <15
hours |
Refer to procedure for
proper protocol; repeat test using fresh specimens |
| Incubator temperature
and/or CO2 content is out of range |
Adjust incubator to
37C / 5% CO2 and repeat the test using fresh specimens |
| The ATP value may be
representative for the individual chosen as the control |
Choose another source
for control specimen and repeat test using fresh specimens |
